环氧合酶-2和血管内皮生长因子mRNA在皮肤鳞状细胞癌组织中的表达

mRNA expression of cyclooxygenase-2 and vascular endothelial growth factor in patients with squamous cell carcinoma

目的探讨环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)mRNA在皮肤鳞状细胞癌(SCC)和正常人皮肤组织中的表达以及它们之间相互关系。方法应用逆转录-聚合酶链反应(RT-PCR)方法,检测24例SCC和10例正常人皮肤组织中COX-2和VEGF mRNA的表达。结果RT-PCR结果显示,在正常人皮肤组织中COX-2和VEGF mRNA呈较弱表达或无表达,吸光度平均值分别为(0．01±0．01)和(0．02±0．02)；有79．2%(19/24)SCC组织中COX-2 mRNA表达水平增高,平均值为(0．56±0．48),与正常皮肤组织比较差异有统计学意义(P＜0．05)；VEGF mRNA在SCC组织中全部高表达(24/ 24,100%),平均值为(0．66±0．35),与正常人皮肤组织比较差异有统计学意义(P＜0．05)。经相关性分析两者之间的表达呈明显正相关(r=0．86)。结论COX-2可能与SCC血管形成有关,且其作用可能通过上调VEGF通道来发挥作用。

Objective To investigate the mRNA expression of cyclooxygenase-2 （ COX-2 ） and vascular endothelial growth factor （ VEGF ） in skin tissue of squamous cell carcinoma （ SCC ） and normal skin tissue, and the relationship between the expression of COX-2 and VEGF. Methods The mRNA expression of COX-2 and VEGF was measured by reverse transcription-polymerase chain reaction （ RT-PCR ） in skin specimens from 24 cases of SCC and 10 normal controls. Results The mRNA expression of COX-2 was higher in SCC specimens than in control skin （ 0.56±0.48 vs. 0.01±0.01, P 〈 0.05 ）. The mRNA expression of VEGF was significantly higher in SCC specimens than in normal skin tissue （ 0.66±0.35 vs 0.02±0.01, P 〈 0.05 ）. And the expression of COX-2 was associated （γ= 0.864 ） with the expression of VEGF. Conclusion COX-2 may be related to the angiogenesis in SCC, and likely to play its role by up-regulation of VEGF expression.