摘要
文章研究了紫叶黄栌组织培养过程中，取材时期、灭菌时间、培养基中附加物种类和浓度对外植体褐变的影响。结果表明，1月取休眠枝条水插培养后抽生的嫩茎作外植体，经0．1％HgCl2灭菌2～3min，外植体褐变程度最轻。培养基中添加1．0mg·L^-1Vc可以有效抑制褐变的发生。
The effects of different periods, sterilization time, various kinds and levels of additives in the medium were studied on browning of explants in tissue culture of Cotinus coggygria ‘ purpureus＇. The results indicate that the optimum explant is the infant shoots sprouting from dormant buds after cultured in water in January, which browns the lightest after sterilized in 0.1% HgCl2 for 2 - 3 minutes. It can repress browning effectively to add 1.0 mg· L^-1 Vc in the medium.
出处
《辽宁林业科技》
北大核心
2006年第4期14-15,20,共3页
Liaoning Forestry Science and Technology
关键词
紫叶黄栌
外植体
褐变
Cotinus coggygria ‘purpureus＇
explant
browning
