摘要
以木瓜蛋白酶水解比色法测定刺参中岩藻聚糖的含量。水解液经次甲基蓝染色,在560nm处有最大吸收波长。吸光度与刺参中岩藻聚糖含量成正比。测定的刺参酸性粘多糖回归方程:y=-0.0189+0.0345x;r=0.9991。并研究了6种不同产地的刺参中岩藻聚糖的含量。
Hydrolization method with papain and colorimetric analysis were used for determining the content of fucoidan in Stichopus japonicus. On the basis of absorbance and concentration, the regression equation was acquired, viz. y=-0. 018 9＋0. 034 5 x, r=0. 999 1. In addition, acid mucopolysacharide in 6 Stichopus from different areas was discussed.
出处
《海洋水产研究》
CSCD
北大核心
2006年第4期44-47,共4页
Marine Fisheries Research
基金
国家科技攻关计划项目(2002BA906A39)资助
关键词
刺参
岩藻聚糖
次甲基蓝
含量
Stichopus japonicus Fucoidan Methylene blue Content
