颅内注射c-fos反义寡聚核苷酸对急性期脑出血周边组织的DNA修复基因表达的影响

Effect of c-fos antisense oligonucleotide on DNA repair gene expression around hematoma in intracerebral hemorrhage

目的:研究c-fos基因对于急性期脑出血周边组织的影响及其作用机制。方法:采用在血肿中心微量注射、免疫组织化学法、原位末端标记的方法在大鼠脑出血模型上观察c-fos反义寡脱氧核苷酸(an tisense ob ligodeoxynucleotides,an tisenseODN s)对DNA修复基因表达的影响。结果:an tisense ODN s能够抑制脑出血周边组织的c-fos阳性细胞表达(P<0.01),加重了血肿周边神经元的损伤。同时,ERCC 1的表达也有下降的趋势(P<0.01),而且在c-fos表达的区域也有ERCC 1的表达。结论:c-fos基因对急性期脑出血周边神经元保护作用可能与脑细胞内源性的DNA修复有关。

Ob.jective： To explore the possible role of c-fos gene involved in neuronal protection, at early stage intracerebral hemorrhage （ICH）. Methods： We used microinjection in hematoma, immunohistochemistry and an terminal deoxynudeotidy tranferase mediated d-UTP- floursceinend- labeling（TUNEI.） assay to observe the effect of c-fos antisense oligonucleotide on the DNA repair gene expression. Results： Antisense oligonucleotide inhibited the expression of c-fos positive cells （P 〈 0. 01） ,and aggravated injury around hcmatoma in ICH, and meanwhile, a decreased tendency to express ERCC was observed in the same region afterantisenseoligonucleotide treatment （P〈0.01）. Furthermore, the distribution of c-fos gene expressed cells was parallel to that of ERCC1 positive cells. Conclusion： The neuroprotective effect of c-fos gene might be related to endogenous DNA repair in the brain cells.