HO-1mRNA在肺癌中的表达

HO-1 mRNA expression in lung cancers

目的:探讨HO-1在肺癌组织中的表达状况,为肺癌的早期诊断提供新的依据。方法:采用反转录-多聚酶链式反应(RT-PCR)方法检测32例单纯肺癌组及10例正常对照组织(距癌组织大约10cm)中HO-1mRNA表达水平。结果:32例标本均进入半定量结果分析,在肺鳞癌组、腺癌组织及肺小细胞未分化癌组HO-1mRNA表达量分别为(0.85±0.03)、(0.84±0.03)及(0.83±0.02),正常对照组为(0.39±0.02)。各型肺癌组HO-1mRNA明显高于正常对照组(P<0.05),而肺癌组间无显著性差异(P>0.05)。结论:HO-1在肺癌中明显的高表达。

Objective： To investigate the expression of HO-1 mRNA in lung cancers to offer new basis for early diagnosis of lung cancers. Methods： Reverse transcription-polymerase chain reaction（RT -PCR） was used to detect HO-1 mRNA expression in specimens from 32 cases of lung cancers and 10 cases of normal tissue about 10cm away from lung cancer tissue. Results： All the 32 specimens were involved in the analysis of half quantity results. The HO-1 mRNA contents were （0.85±0.03）, （0. 84± 0. 03）and（0. 83±0. 02）in squamous cell carcinoma of the lung, adenocarcinoma of the lung and small cell anaplastic lung cancers. The HO-1 mRNA content was （0. 39±0. 02） in normal tissue. HO-1 mRNA expression rate was higher in lung cancers than that normal tissue （P 〈 0.05） . There was no significant difference among all types of lung cancers （P 〉 0.05） . Conclusion. HO-1 mRNA quantity shows much high expression in lung cancers.