垂柳组织培养初步研究

The preliminary study on tissue culture of Salix bfabylonica

以垂柳嫩枝条为外植体进行茎段组织培养试验研究。结果表明:垂柳茎段最适启动培养基及激素配比为1/2MS+6-BA 1.0 mg/L+NAA 0.05 mg/L+1.5%蔗糖;最适不定芽诱导培养基及激素配比为WPM+6-BA0.1 mg/L+NAA 0.2 mg/L+3%蔗糖;不定根最适诱导培养基为1/2MS+NAA 0.05 mg/L+1.5%蔗糖。

Induction of stem of Salix baby/on/ca Linn was study.The result indicated that 1/2MS＋ 6- BA 1.0 mg/L＋ NAA 0.05 mg/L ＋ 1.5% sucrose was the best basic medium in initiated culture;the optimal medium of adventitious buds differentiation was WPM ＋ 6- BA 0.1 mg/L ＋ NAA 0.2 mg/L ＋ 3 % sucrose;and the optimal medium of adventitious roots was 1/ 2MS＋ NAA 0.05 mg/L＋ 1.5% sucrose .