摘要
对大花蕙兰茎尖进行了组织培养研究。试验结果表明:原球茎诱导、增殖和生根培养基分别是MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+香蕉汁100 g/L+活性炭0.5 g/L,MS+6-BA 1.5 mg/L+NAA 0.5 mg/L+香蕉汁130 g/L+活性炭1.0 g/L,1/2 MS+6-BA 0.1 mg/LAA 0.5 mg/L+香蕉汁150 g/L+活性炭1.5 g/L。
The tissue culture of Cymbidum were studied by using shoot - tip. The results showed that the explants cultured on MS + 6 - BA 1.0 mg/L+ NAA 0.1 mg/L+ banana 100 g/L+ carbon 0.5 g/L, and MS + 6 - BA 1.5 mg/L + NAA 0.5 mg/L+ banana 130 g/L+ carbon 1.0 g/L,and 1/2 MS+ 6- BA0.1 mg/L+ NAA 0.5 mg/L+ banana 150 g/L+ carbon 1.5 g/L were suitable for inducing PLBs,muhiplicating PLBs and rooting of microshoots,respectirely.
出处
《河北林果研究》
2006年第3期278-280,共3页
Hebei Journal of Forestry and Orchard Research
基金
河南省重点科技攻关项目(0423020700)
关键词
大花蕙兰
组织培养
原球茎
Cymbidum
tissue cuhure
protocorm- like- hodies(PLBs)