大花蕙兰组织培养的研究

Studies on tissue culture of Cymbidum

对大花蕙兰茎尖进行了组织培养研究。试验结果表明:原球茎诱导、增殖和生根培养基分别是MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+香蕉汁100 g/L+活性炭0.5 g/L,MS+6-BA 1.5 mg/L+NAA 0.5 mg/L+香蕉汁130 g/L+活性炭1.0 g/L,1/2 MS+6-BA 0.1 mg/LAA 0.5 mg/L+香蕉汁150 g/L+活性炭1.5 g/L。

The tissue culture of Cymbidum were studied by using shoot - tip. The results showed that the explants cultured on MS ＋ 6 - BA 1.0 mg/L＋ NAA 0.1 mg/L＋ banana 100 g/L＋ carbon 0.5 g/L, and MS ＋ 6 - BA 1.5 mg/L ＋ NAA 0.5 mg/L＋ banana 130 g/L＋ carbon 1.0 g/L,and 1/2 MS＋ 6- BA0.1 mg/L＋ NAA 0.5 mg/L＋ banana 150 g/L＋ carbon 1.5 g/L were suitable for inducing PLBs,muhiplicating PLBs and rooting of microshoots,respectirely.