儿童病毒性脑炎脑脊液白细胞介素-8含量测定及评价

Evaluation and measurement of IL-8 in cerebrospinal fluid of viral encephalitis in children

目的检测病毒性脑炎患儿脑脊液(CSF)白细胞介素-8(IL-8)浓度并探讨其临床意义。方法病毒性脑炎患儿43例,其中重症组26例,轻症组17例,对照组16例。用酶联免疫吸附法(ELISA)检测各组患儿脑脊液IL-8含量,并做比较分析。结果轻症组及重症组脑脊液IL-8水平明显高于对照组(P<0.05);而重症组脑脊液IL-8水平又明显高于轻症组(P<0.001)。结论病毒性脑炎患儿脑脊液IL-8含量明显升高,以重症组升高明显,提示IL-8在病毒性脑炎发病机制中起重要作用,可作为病毒性脑炎诊断的辅助指标之一,有助于衡量病情轻重。

Objective To observe the levels and clinic significance of interleukin-8（IL-8） in cerebrospinal fluid（CSF） of infants suffering from viral encephalitis（VE）. Methods Among 43 confirmed VE cases, 23 cases were male and 20 cases were female, with average age of 5.9 years. Then there were 26 severe VIE cases and 17 mild VE cases. 16 children with no-CNS-infection were used as contrast group. One sample of CSF was taken from each children. All sample were kept at - 20℃. The levels of IL-8 in CSF were measured with sandwich ELISA methods, and the ELISA kits were provided by the company of JinMei Biotech in BeiJing. Results The levels of IL-8 in VE group were higher than that of contrast group（P〈0.05）. Level d IL-8 d severe group was especially higher than that of contrast group（ P 〈 0.001 ） , and much higher than that of mild group（ P 〈 0.001）. Conclusion IL-8 not only gives contribution in anti-viral dfect,but also plays a role in pathogenesis of encephalitis in children. It suggests the levels of IL-8 in CSF can be used as one of indicators evaluating tendency of the VE.