高效液相色谱法测定人血浆中阿糖胞苷的浓度

Determination of Cytarabine in Human Plasma by HPLC

目的：建立以高效液相色谱法测定人血浆中阿糖胞苷浓度的方法。方法：色谱柱为Zorbax C18，检测波长为280nm，流动相为0．01mol／L磷酸盐（pH=7．0）-乙腈（95：5），流速为1．0ml／min。结果：阿糖胞苷血药浓度在0．05～20μg／ml（r=0．9999）范围内线性关系良好，最低检测浓度为2ng／ml，方法回收率为98．3％～103．2％，日内、日间相对标准差分别为2．8％、3．6％。结论：本方法简单、快速、准确，适用于阿糖胞苷临床血药浓度监测和药动学研究。

OBJECTIVE：To establish a HPLC method for the determination of cytarabine in human plasma. METHODS： The assaying was performed on Zorbax-C18 column,the mobile phase consisted of 0.01mol/L sodium phosphate （pH = 7.0） - acetonitrile（9S ： S）with flow rate at 1.0ml/min and UV detection wavelength at 280nm .RESULTS： The linear blood concentration range for cytarabine was 0.05～20μg/ml（ r = 0.9 999）with the minimum detection limit at 2ng/ml, the method recovery was 98.3% ～ 103.2% , the relative standard deviations of intra - day and inter day were 2.8% and 3.6% , respectively.CONCLUSIONS：This method was simple, fast and accurate,and suitable for the assaying of cytarabine in human plasma and pharmacokinetics study.