大孔吸附树脂分离纯化甘草总黄酮工艺研究

Studies on Separation and Purification of Total Flavones from Licorice by Macroreticular Adsorptive Resin

目的:研究大孔吸附树脂分离纯化甘草总黄酮的工艺条件。方法:比较D101、Hz-806、AB-83种大孔吸附树脂对甘草总黄酮的静态解吸率,然后通过对甘草醇提取液上的大孔吸附树脂柱,以不同浓度乙醇依次洗脱,并考察洗脱液中甘草总黄酮的含量、固形物重量和纯度。结果:AB-8型解吸率最好,具体工艺参数为上样速度3ml/min、药液浓度1.5mg/ml、pH值5,及80%乙醇为洗脱剂。结论:AB-8型大孔吸附树脂能有效分离纯化甘草总黄酮,纯度大于50%并,符合中药有效部位研究要求。

OBJECTIVE： To establish a technical process for the separation and purification of total flavones from Licorice. METHODS： The static absorption capacity of macroreticular adsorptive resins D101. Hz - 806. AB - 83 for total flavones from licorice were compared. The macroreticular adsorptive resin columns on the Licorice extractives were eluted respectively with different concentrations of ethanol, then the content, the weight of residue and purity coefficient of flavones from Licorice in the eluant were detected. RESULTS： The optimal technological conditions were found in AB- 8 as follows： flow rate= 3ml/min, sample concentration = 1.5 mg/ml, pH= 5 and 80% ethanol was used as eluting agent. CONCLUSIONS： AB- 8 macroreticular adsorptive resin can effectively separate and purify total flavones from licorice, the purity coefficient thus obtained being over 50%, which meets the requirement of the study of effective components of herbal medicine.