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小鼠Oct4基因的克隆及其原核表达载体的构建 被引量：2

Cloning of Mouse Oct4 Gene and Construction of Expression Vector
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摘要 Oct4是POU区转录因子家族中的一员,在未分化胚胎癌和胚胎干细胞中表达.对细胞未分化状态的维持发挥重要作用。本文通过RT-PCR方法克隆得到小鼠Oct4基因,并成功构建其原核表达载体pET-15b-Oct4,为今后对其功能及生物学活性的进一步研究奠定了基础。 Oct4 is a mammalian POU transcription factor expressed by early embryo cells and germ cells . It＇s important in keeping the undifferentiated state of ES cell. We have accurately cloned mouse Oct4 gene fragment by RT-PCR and constructed the pET-15b- Oct4 vectors. The cloning of mouse Oct4 gene and construction of it＇s expression vectors lay a good foundation of further study on the function, biological activity and application.
作者 宋凯 宋继梅 张涌
机构地区 西北农林科技大学生物工程研究所
出处 《西北农业学报》 CAS CSCD 北大核心 2006年第5期10-13,共4页 Acta Agriculturae Boreali-occidentalis Sinica
基金 国家"863"项目资助(2001AA213081)
关键词 小鼠 OCT4 基因克隆 原核表达载体 Mouse Oct4 Gene cloning Expression vector
分类号 Q78 [生物学—分子生物学]
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参考文献9

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同被引文献36

  • 1林艳丽,曹立雪,吴晓洁,阎新龙,邓继先.小鼠Nanog基因的克隆及其在大肠杆菌中的表达[J].中国生物工程杂志,2005,25(B04):257-259. 被引量：5
  • 2Scholer HR.Octamania:The POU factors in murine development. Trends Genet 1991;7(10):323-329
  • 3Enst M, Oates A, Dann AR. Gp130-mediated signal transduction in embryonic stem cells involves in activation of JAK and Ras/mitogen activated protein kinase pathways. J Biol Chem 1996;271(47):30136-43
  • 4Ying QL, Nichols J, Chambers I, et al. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3.Cell 2003;115(3):281-292
  • 5Chambers I.The molecular basis of pluripotency in mouse embryonic stem cells. Cloning Stem Cells 2004;6(4):386-391
  • 6Cavaleri F, Sch?ler HR. Nanog:a new recruit to the embryonic stem cell orchestra. Cell 2003;113(5):551-552
  • 7Loh YH, Wu Q, Chew JL, et al. The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 2006;38(4):431-440
  • 8Boiani M, Gentile L, Gambles VV, et al. Variable reprogramming of the pluripotent stem cell marker Oct4 in mouse clones: distinct developmental potentials in different culture environments.Stem Cells 2005;23(8):1089-1104
  • 9Boyer LA, Lee TI, Cole MF,et al.Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 2005;122(6):947-956
  • 10Lavial F, Acloque H, Bertocchini F,et al. The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells. Development 2007;134(19): 3549-3563

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西北农业学报
2006年 第5期

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