摘要
以甘蔗花叶病毒(SCMV)福建分离物(FJ)的总RNA为模板,用RT—PCR方法扩增了含有复制酶(NIb)基因的DNA片段,将其克隆到质粒pGM—T载体上并进行了序列分析。结果表明,NIb基因由1563个碱基组成,编码521个氨基酸并含有高度保守序列GDD。NIa/NIb和NIb/CP交界处的蛋白酶切割位点分别为Q/C和Q/A。与国外报道的SCMV亚组几个株系分离物相比,NIb基因(福建分离物)的核苷酸和氨基酸序列同源性分别为57.8%~86.9%和60.9%~95.2%,其中与SCMV—SA株系的核苷酸及氨基酸序列同源性则高达86.9%和95.2%。
The NIb coding region of sugarcane mosaic virus (SCMV) from Fujian was amplified from the extracted plant total RNA by using RT-PCR, and cloned into the pGM-T vector and sequenced. The result indicated that the NIb coding region comprised 1563 nucleotides and encoded a putative protein of 521 amino acids containing the consensus motif GDD. The protease cleavage sites between the NIa/NIb and NIb/coat protein were found to be Q/C and Q/A respectively. The nucleotide and the putative amino acid of replicase were compared with the other isolates or stain of SCMV subgroup, it shares 57.8%-86.9% and 60.9%-95.2% homology in nucleotide and the putative amino acid sequence respectively. The NIb sequence was most similar to SCMV-SA with identities of 86.9% and 95.2 % at the nucleotide and amino acid levels respectively.
出处
《西北农业学报》
CAS
CSCD
北大核心
2006年第5期80-84,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家863高技术研究发展计划项目资助(2002AA241031)
湖北省教育厅青年基金项目资助(Q200513002)
关键词
甘蔗
花叶病毒
复制酶因
序列测定
Sugarcane
Mosaic Virus
Coat Protein
Molecular Detection