同心盾壳霉产孢条件研究

Study on Sporulation of Coniothyrium concentricum

对同心盾壳霉产孢条件研究结果表明：该病原菌在PSA和PDA上产孢量最大，而在玉米粉琼脂培养基、胡萝卜煎汁培养基和V8培养基上不产生分生孢子器；在15℃～35℃下，该病原菌均可产孢，在30℃下产孢量最大；产孢的最佳碳源为蔗糖，其次为乳糖和葡萄糖；无机氮源不能满足病原菌产孢。产孢的最适pH为6～8，偏酸条件比偏碱下产孢量稍大；黑暗有利于病原菌产孢，紫外线照射对其产孢影响不大；

Researched on sporulation quantity of Coniothyrium concentricum, the results showed that： sporulation quantity of Coniothyrium concentricum on culture medium of PSA and PDA was higher than others,but no spores reproduced when the pathogen grew on culture of corn ,carrot and vS. Temperature from 15℃ to 35℃ ,the pathogen could spore and the optimum temperature for sporulation was 30℃. The optimum carbon for sporulation was sucrose,secondly was lactose and dextrose. Abio-nitrogen could not satisfy sporulation. The optimum pH was 6-8, sporulation of the pathogen in the condition of partial acid was higher than it in the condition of patrial alkali. Darkness was benefit sporulate. Ultraviolet radiation was no significant effect on sporulation.