铁皮石斛原球茎多糖提取最佳工艺研究

Studies on the Optimum Technology for Extraction of Polysaccharides from Suspension-cultured Protocorms of Dendrobium candidum

对铁皮石斛悬浮培养原球茎多糖的提取、纯化条件进行优化研究,正交试验结果表明,提取的最佳工艺为80℃热水中浸提2 h,加水量20倍,提取3次,醇析时乙醇的浓度为80%。粗多糖脱蛋白时,氯仿/正丁醇(v/v)为1∶0.4,样品/氯仿+正丁醇(v/v)为1∶0.35,萃取时间采用5 min最佳。

Conditions of the extraction and purification of polysaccharides from suspension-cultured protocorms of Dendrobium candidum were optimized by orthogonal experiment. The results showed that the optimum technology of isolation is to extract about 3 times and 2 hours each time in 20 times water at 80℃, to precipitate polysaccharides with 80 % ethanol, and to remove proteins when chloroform/n-butanol is 1 ： 0.4, the sample/ chloroform-n-butanol is 1 ： 0.35 and the abstracting time is 5 min.