发根农杆菌诱导黄芩毛状根的研究

Research of Hairy Roots Induced Scutellaria baicalensis Georgi by Agrobacterium rhizogenes

利用发根农杆菌A4,A4 9615,1.2556诱导中药黄芩外植体,得到黄芩不定根,根据形态和在MS培养基中进行培养证实得到黄芩毛状根,经抗菌素氨苄西林钠500 mg/L除菌后,毛状根表现出分枝多,生长快,且能在MS中生长繁殖的特性,为采用生物技术生产黄芩活性成分奠定基础。

Hairy roots were induced from Scutellaria baicalensis Georgi by three kinds of Agrobacteriurn rhizogenes strains A4, A4 9615 and 1. 2556. They were revealed by appearance and growing on hormone-free medium. There are some significant characteristics of hairy roots after eliminating bacteria with antibiotic, such as more branches, fast growth capacity and growth in hormone-free media. It is the basis for producing active components of Scutellaria baicalensis Georgi by biotechnology.