摘要
用成人外周血T淋巴细胞总E花环法、正常成人血T淋巴细胞45C脱E受体的E花环法以及脐血淋巴细胞E花环法,进行HPIF免疫活性检测的比较研究,结果表明用正常成人外周血淋巴细胞45C脱E受体的E花环法最佳。建议将此法作为HPIF免疫活性检测的常用方法。
A comparison was made among the four methods for the determination of immunological activity of human placenta immunoregulatory factor (HP1F). E-rosette formation test (ERFT) with adult peripheral blood lymphocytes (PBL), ERFT with normal adult PBL which heated at 45 C for 1h, ERFT with umbilical cord blood lymphocytes. These results show that ERFT with normal adult PBL which heated at 45 C for1h is the best method among our studies, We suggust that this assay could be used as routine method in the determination of the immunological activity of HP1F.
出处
《中国生化药物杂志》
CAS
CSCD
1996年第5期205-206,共2页
Chinese Journal of Biochemical Pharmaceutics