阿尔茨海默病的调节性T细胞功能检测

T cells regulatory function in Alzheimer's Disease

目的对阿尔茨海默病(AD)患者外周血调节性T细胞功能进行研究。方法流式细胞仪测定T细胞亚群;细胞分选仪分离调节性T细胞,体外培养行淋巴细胞混合培养法检测调节性T细胞对同源/异源淋巴细胞增殖的抑制效应;ELISA法检测调节性T细胞细胞因子肿瘤坏死因子-β、白细胞介素-10的分泌水平。结果CD4+CD25+调节性T细胞细胞比例和健康对照组相比差异无显著性。肿瘤坏死因子-β、白细胞介素-10的分泌2组间差异无显著性。AD组调节性T细胞抑制功能受损,与对照组有明显差异。结论AD患者调节性T细胞抑制功能受损,可能与其接触抑制途径缺陷有关。

Objective To investigate the suppressive function of T regulatory cell in Alzheimer＇s disease （ AD ） patients. Methods The T lymphocyte subset was detected by flow cytometery. The CD4 ^＋ CD25 hi T regulatory cells were purified by eell sorter. The secretion of TNF-β, and IL-10 were measured by ELISA. The direct suppression effect of CD4 ^＋ CD25hi T regulatory cells on T lymphoeytes proliferation were performed by the mixed lymphoeytes reaction and measured by 3H-Thymidine radioactive assay. Results No significant differences were found in T lymphocyte subpopulations, including CD4 ^＋ CD25hi T regulatory cells between groups. No significant differences were found in TNFβ, and IL-10 secretion. A significant decrease was found in the function of CD4 ^＋ CD25 hi T regulatory cells from peripheral blood of patients with AD as compared with healthy donors （ P 〈 0.05 ）. Conclusion The decreased suppressive effect may be related with the deficit in the pathway of cell-to-cell contact.