用SSR标记构建家蚕中系品种资源指纹图谱

Construction of Fingerprints of Silkworm Germplasm Resources Based on SSR Markers

利用微卫星标记SSR(Simple Sequence Repeat),在DNA水平上构建了96个中国系统二化性家蚕品种的DNA指纹图谱。57对SSR引物共扩增出507条有效带,最多的一对引物扩增出了24个片段,最少的仅有1个,平均为8.9个/引物,表明各品种之间存在丰富的微卫星多态性。家蚕品种间遗传距离最大值为0.2572,最小值为0.095,根据每个品种间的遗传距离,利用UPGMA聚类法进行了聚类分析,发现SSR扩增片段具有品种特异性,能准确地对各品种进行分类和鉴别。

The fingerprints of 96 Chinese bivoltinism silkworm races are constructed by using the microsatellite （SSR） markers. 507 valid bands are amplified through 57 pairs of SSR markers. The number of the bands about single pair of markers varied from 1 to 24, and the mean value is 8.9/primer, indicating that the microsatellite （SSR） markers can reflect the polymorphism among diverse silkworm races. The maximum of genetic distance is 0. 2572 while the least is 0. 0951. A dendrogram is obtained by the UPGMA method using the total number of amplified SSR fragments. Results show that the specific SSR markers can unambiguouslydistinguish the different bivoltinism silkworm races of Chinese system.