南方红豆杉中产紫杉醇内生真菌的分离和筛选

Isolation and Screening of Endophytic Fungi Synthesizing Taxol from Taxus chinensis var.mairei

从南方红豆杉中分离得到21株内生真菌,在PDA液体培养基中发酵后经HPLC检测,发现有3株能够产生紫杉醇,它们是XT5(Ectostromasp.)、XT2(botrytissp.)和XT17(Papulasporasp.),其紫杉醇产率分别是276.75μg/L、161.24μg/L和10.25μg/L.

Twenty-one endophytic fungi have been already isolated from Taxus chinensis var. mairei. Three strains＇ cultures have been checked out taxol by HPLC in these endophytic fungus. The taxol yield of XT5 （Ectostroma sp.）, XT2（botrytis sp. and XT17（Papulaspora sp.）were 276.75 μg/L, 161.24 μg/L, and 10.25 μg/L, respectively.