水溶性氮酮对人眼小梁细胞增殖活性与形态结构的影响

Effects of Water Soluble Azone on the Proliferation and Structure of Human Trabecular Mashwork Cells

目的观察经皮渗透促进剂氮酮对体外培养的人眼小梁细胞增殖活性和超微结构的影响，寻找氮酮作用于眼部的安全浓度。方法进行人眼小梁细胞的体外培养，四氮唑盐（MTT）法观察不同浓度氮酮作用不同时间对小梁细胞增殖的影响；倒置相差显微镜和透射电镜下观察氮酮对小梁细胞形态和超微结构的影响；荧光染色法观察氮酮对小梁细胞活性的影响。结果10^-8～10^-5g·L^-1氮酮作用24h不影响小梁细胞的增殖。10^-8～10^-6g·L^-1氮酮对小梁细胞形态和超微结构无影响。荧光染色法显示氮酮作用后的细胞呈不同程度变性坏死。结论10^-8～10^-6g·L^-1氮酮不会影响小梁细胞的形态结构和增殖活性，低浓度氮酮在理论上对小梁细胞是安全的。

Objective To study the influence of water soluble azone on proliferation and structure of human trabecular mashwork cells （HTMC）. Methods Human trabecular mashwork cells were cultured in vitro and treated with different concentrations of azone. MTT assay, transmission electron microscope and acridine orange fluorescent staining were used to observe the influence of azone on the cells＇ vitality and uhrastructure. Results Azone, 10^-8 - 10^-5g·L^-1, had no significant influence on the proliferation of HTMC after acting for 24 h and 10^-8 - 10^-6g·L^-1 of water soluable azone also had no significant influence on the uhrastructure of human trabecular mashwork cells. Acridine orange fluorescent staining showed that those cells treated with 10^-3 g·L^-1 or 10^-4 g·L^-1 of azone lost its vitality in some degree. Conclusion Water soluable azone, 10^-8 - 10^-6g·L^-1, had no significant influence on the proliferation, vitality and uhrastructure of human trabecular mashwork cells. Low concentration of azone incorporated into the ophthalmic formulations of drugs is safe to human trabecular mashwork cells in theory.