摘要
目的探讨在中国人群中组织因子途径抑制物基因第9外显子874G→A(V264M)多态性与急性冠状动脉综合征的关系,以及各基因型对血浆游离组织因子途径抑制物水平和冠状动脉病变严重程度的影响。方法选取经冠状动脉造影检查的136例急性冠状动脉综合征患者和106例健康对照者,用聚合酶链反应和限制片长多态性方法分析组织因子途径抑制物(V264M)基因型。利用酶联免疫吸附试验检测部分患者血浆游离组织因子途径抑制物水平。结果急性冠状动脉综合征组血浆游离组织因子途径抑制物水平明显高于正常组(P<0.05),两组的V264M基因型和等位基因的分布趋势相同,差异无显著性(P>0.05)。A等位基因携带者的游离组织因子途径抑制物水平低于GG基因型者(6.9±5.1比14.3±9.5),而冠状动脉病变程度不受V264M基因多态性的影响(χ2=0.413,P>0.05)。结论中国人群中存在V264M多态性,它对血浆游离组织因子途径抑制物水平可能有影响,但未发现V264M多态性与急性冠状动脉综合征发病存在相关性。
Aim To investigate TFPI V264M polymorphism in Chinese and its effect on plasma levels of free tissue factor pathway inhibitor（f-TFPI）and seventy ot acute coronary syndrome （ACS）. Methods The genotypes ot V264M were detected by PCR and PCR-RFLP in 136 ACS patients and 106 controls undergoing coronary angiography, then the serum f-TFPI level in 57 cases was inspected by enzyme linked immunosorbent assay. Results The allele and genotype frequencies were consistent with that predicted by Hardy weinberg equilibrium in the present study population （χ^2 = 0.437, P〉0.05）. Plasma f-TFPI level was significantly lower among carriers of Aallel as compared with non-carriers （ GG genotype ） [ 6.9 ± 5.1 ） vs （ 14.3 ± 9.5 ） ]. ACS group showed significantly higher plasma level of f-TFPI than control group （ P〈0.05 ）. No meaningful corelationship was found between ACS group and control group for V264M polmorphism （ P〉0.05 ）. Conclusion The V264M polymorphism do have an effect on the serume f-TFPI level but have no relationship with the occurrence and the severity of ACS.
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第1期64-66,共3页
Chinese Journal of Arteriosclerosis
