血脂康对载脂蛋白E基因敲除小鼠肝细胞内Ca^(2+)及线粒体膜电位的影响

Effects of Xuezhikang on Ca^(2+) and Mitochondrial Membrane Potential in Hepatocytes of Apolipoprotein E Gene-Knock Out Mice

目的观察血脂康胶囊对载脂蛋白E基因敲除小鼠肝细胞内Ca2+浓度及线粒体膜电位的影响。方法采用胶原酶消化法分离载脂蛋白E基因敲除小鼠肝细胞,体外原代培养8天后,加入含10%血脂康血清的培养基,48 h后以Flou-3/AM和JC-1为探针,应用激光共聚焦显微镜测量肝细胞内Ca2+浓度和线粒体膜电位。结果血脂康可明显降低载脂蛋白E基因敲除小鼠肝细胞内Ca2+浓度,与对照组比较差异有显著性(P<0.01);同时,血脂康可明显提高载脂蛋白E基因敲除小鼠肝细胞线粒体膜电位(P<0.05)。结论血脂康可降低载脂蛋白E基因敲除小鼠肝细胞内Ca2+浓度,提高线粒体膜电位,这可能是血脂康对抗高脂血症发生和发展的重要机制之一。

Aim To observe the effects of Xuezhikang on Ca^2＋ concentration and mitoehondrial membrane potential in hepatocytes of apolipoprotein E gene-knock out mice. Methods The hepatocytes of APOE^-/- mice were separated with collagenase and cultured in RPMI1640 with 10% fetal bovine serum for 8 days. Then the hepatocyes were incubated in culture medium with 10% Xuezhikang-containing rat serum for 48 hours. Finally, after stained with flou-3/AM and JC-1, the Ca^2＋ concentration and the mitochondrial membrane potential in hepatocytes were detected with laser scanning confocal microscope. Results Xuezhikang could decrease Ca^2＋ concentration in hepatocytes and there was significant difference between Xuezhikang group and control group（ P 〈 0.01 ）. Xuezhikang could also improve mitochondrial membrane potential in hepatoeytes （ P 〈 0. 05 ）. Conclusion Xuezhikang can decrease the Ca^2＋ concentration and improve mitochondrial membrane potential in bepatocytes. ＂lifts may be important mechanisms of Xuezhikang in anti-hyperlipemia.