巨噬细胞集落刺激因子对RAW264.7细胞基质金属蛋白酶9分泌的影响

Effects of Macrophage Colony-Stimulating Factor on Protease Secretion from RAW264.7 Cell

目的探讨巨噬细胞集落刺激因子致动脉粥样硬化作用是否与其影响基质金属蛋白酶表达和活性有关。方法应用酶谱法观察巨噬细胞集落刺激因子对体外培养的RAW264.7细胞基质金属蛋白酶9活性的影响;逆转录聚合酶链反应检测巨噬细胞集落刺激因子对体外培养的RAW264.7细胞基质金属蛋白酶9 mRNA表达的影响。结果与对照组相比,不同浓度(100、250和500μg/L)巨噬细胞集落刺激因子能增强RAW264.7细胞基质金属蛋白酶9的活性,并呈一定的剂量依赖性;同时,250μg/L巨噬细胞集落刺激因子可增强RAW264.7细胞基质金属蛋白酶9 mRNA表达。结论巨噬细胞集落刺激因子可诱导RAW264.7细胞基质金属蛋白酶9的表达,并可能通过这一机制影响动脉粥样硬化斑块的稳定性。

Aim To investigate the effect of macrophage colony-stimulating factor（M-CSF） on the expression and activity of matrix metalloproteinases （MMP） in RAW264.7 cell. Methods Gelatin zymography analysis was used to detect the effect of M-CSF on activity of MMP-9 in cultured RAW264.7 cell. Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the effect of M-CSF on expression of MMP-9 mRNA in cultured RAW264.7cell. Results The enzyme activity of MMP-9 was significantly increased after 24 h M-CSF treatment. M-CSF had no effect on the activation of MMP-9 secreted. RT-PCR showed that M-CSF upregulated MMP-9 mRNA expression after 8-hour M-CSF treatment. Contusion M-CSF upregulates MMP-9 expression in RAW264.7 cell, which may play an important role in plaque destabilization.