肥胖者高密度脂蛋白亚类组成与脂蛋白酯酶基因多态性的关系

Relationship between High Density Lipoprotein Subclasses Distribution and Lipoprotein Lipase Gene HindⅢ Polymorphism in Obese Subjects

目的探讨肥胖者高密度脂蛋白亚类组成与脂蛋白酯酶基因内含子8 HindⅢ酶切位点多态性的关系。方法采用聚合酶链反应限制性片长多态性和双向电泳免疫印迹检测法,分析95例肥胖者和144例体质指数正常者的脂蛋白酯酶基因内含子8 HindⅢ多态性、高密度脂蛋白亚类组成及相对百分含量。结果肥胖组和对照组均以H+H+基因型为主。肥胖组血浆甘油三酯、载脂蛋白B100、CⅡ、CⅢ、E、前β1高密度脂蛋白水平及甘油三酯/高密度脂蛋白胆固醇较对照组增加,而高密度脂蛋白胆固醇、载脂蛋白AI、高密度脂蛋白2b水平及载脂蛋白E/CⅢ明显降低(P<0.05)。肥胖组H+H+基因型者与H-H-者比较,血清甘油三酯、载脂蛋白B100、前β1-高密度脂蛋白、高密度脂蛋白3b含量显著升高,而载脂蛋白E/载脂蛋白CⅢ、高密度脂蛋白2a和高密度脂蛋白2b显著降低;肥胖组H+H-基因型者与H-H-者比较,载脂蛋白B100、前β1高密度脂蛋白和高密度脂蛋白3b含量显著升高,而载脂蛋白E/CⅢ和高密度脂蛋白2a显著降低(P<0.05)。对照组H+H+及H+H-基因型者血清载脂蛋白CⅡ、载脂蛋白CⅢ和高密度脂蛋白3a含量均较H-H-者显著升高,而载脂蛋白E/载脂蛋白CⅢ、高密度脂蛋白2a、高密度脂蛋白2b显著降低(P<0.05)。结论脂蛋白酯酶基因H+H+基因型与肥胖者增高的甘油三酯水平有关。中国人脂蛋白酯酶基因HindⅢ酶切位点多态性与高密度脂蛋白亚类的组成和分布相关,H+等位基因携带者高密度脂蛋白颗粒有变小的趋势,且肥胖者更为明显,表明H+等位基因携带者高密度脂蛋白成熟代谢障碍。

Aim To elucidate the relationship between hpoprotein lipase （LPL） gene HindⅢ polymorphism and plasma lipid profides and HDL subclasses in obesity. Methods LPL gene Hind Ⅲ polymorphism was assayed by polymerase chain reaction-restriction fragnent length polymorphism （ PCR-RFLP）. The subclasses of serum HDL in 95 obese subjects and 144 nonobese subjects were determined by two-dimensional gel electrophoresis conjunction with immtmodetection method. Results Both in obese group and control group, the H^＋ H^＋ homozygote and H^＋ allele were the major allelotype. There was no statistically significant difference in frequencies of genotypes or alleles between two groups. Obese group had higher plasma triglycerides, apoB100, apoCⅡ , apoCⅢ, apoE, preβ1-HDL levels and TG/HDLC, but lower HDLC, apoAI, HDL2b, apoE/CⅢ compared witli control group （ P 〈 0.05）. In obese group, Hind Ⅲ H^＋ H^＋ had higher plasma triglycerides than H^- H^- genotype. Compared with H^-H^- genotype, the subjects with H^＋ H^＋ and H^＋ H^- genotypes had higher apoB100, preβ1-HDL, HDL3b levels but lower apoE/C Ⅲ I, HDL2a, HDL2b. In control group, the genotypes of H^＋ H^＋ and H^＋ H^- showed higher apoC Ⅱ , apoCⅢ, HDL3b levels, but lower apoE/CⅢ, HDL2a, HDL2b levels compared with the genotype of H^- H^- （ P 〈 0.05）. Conclusions H^＋ H^＋ genotype was associated with higher plasma TG levels of obesity. LPL gene polymorphism was associated with plasma levels as well as associated with changes of HDL subclasses distribution in Chinese population. And the particle size of HDL tended to be smaller in H^＋ allele cartier＇ subjects, furthermore, the tendency was more obvious in obese group, which indicated that HDL maturation might be abnormal in subjects with H^＋ allele.