一个水稻新黄绿叶突变体基因的分子定位

Genetic Analysis and Molecular Mapping of a New Yellow-Green Leaf Gene ygl-2 in Rice

在水稻品种武运粳7号中发现了一个黄绿叶自然突变体,经过多代自交形成了稳定的突变系。该突变系和武运粳7号的正反交F2代的遗传分析表明该材料的黄绿叶由1对隐性基因控制,命名为ygl-2。利用已有的微卫星(SSR)标记和新发展的SSR标记将ygl-2基因定位于RM1340、RM7269、RM6298、SSR6-16和RM7434、SSR6-5、SSR6-9、RM5957之间,排列位置为RM1340-RM7269-RM6298-SSR6-16-ygl-2-RM7434-SSR6-5、SSR6-9-RM5957,它们之间的遗传距离分别为2·38、0.37、0.00、0.62、0.74、0.49、0.86和1.62cM,这为ygl-2基因的分子标记辅助选择育种和图位克隆奠定了基础。

A natural new rice yellow green leaf mutant, derived from a japonica rice variety Wuyunjing 7, was crossed with the wild type variety and an indica rice variety Jiangxi 1587 to investigate the genetic model and map the referring gene（s）. The yellow-green leaf mutant, named ygl-2 following the similar mutant reported before was controlled by a recessive gene ygl-2. The gene was roughly mapped between two microsatellite markers RM541 and RM30, with genetic distances of 13.1 cM and 9.1 cM, respectively. Then eight new polymorphic microsatellite markers were developed in this region. The ygl-2 was further mapped between two microsatellite markers SSR6-16 and RM7434, with genetic distances of 0.62 cM and 0.74 cM, respectively.