适于水稻根、叶、悬浮细胞总蛋白质分析的高分辨率双向电泳方法

High-Resolution Two-Dimensional Electrophoresis for Total Proteins in Rice Roots, Leaves and Suspension Cells

提取了水稻根、叶、悬浮细胞的总蛋白质，用双向电泳进行分离，得到了高分辨率和高重复性的双向电泳图谱。在根、叶、悬浮细胞的双向电泳图谱上能分别检测到至少1190、1320、1080个蛋白点，重复样品图谱间的相关系数在0．87～0．94。还对水稻蛋白质的提取过程和电泳条件进行了改进和优化，对其他植物蛋白的双向电泳分析有一定的借鉴作用。

Total proteins from rice roots, leaves and suspension cells were extracted and separated by two-dimensional electrophoresis. At least 1190, 1320, 1080 protein spots were detected on the gels of root, leaf and suspension cell extracts, respectively. The gels were highly reproducible as determined by the correlation coefficient among replications, which varied from 0.87 to 0.94. The protein, extraction procedure and the electrophoresis conditions were optimized, which may be helpful for proteomic analysis of other plant species by two-dimensional electrophoresis.