近交系大鼠心脏移植模型建立的实验研究

Construction of heart heterotopic transplantation model in inbred strain rat

目的:建立近交系大鼠同种异位腹腔心脏移植动物模型。方法:采用改良的ono术式建立Brown Norway到Lewis大鼠的腹部异位心脏移植,将供心升主动脉、主肺动脉分别与受体腹主动脉、下腔静脉进行端侧吻合。采用分别结扎右上腔和下腔静脉后一次性集束结扎法取心,行端侧血管吻合连续缝合法。结果:共进行了48例大鼠心脏异位移植,经过预实验16只的技术总结,正式实验32只手术成功率为96.88%,供心冷、热缺血时间和心脏复跳时间明显缩短,手术时间减少至60min。结论:加强供心的综合保护和围手术期的处理,是移植模型建立的关键因素。

Objective： To establish the model of inbred strain rat heart heterotopic transplantation. Methods：Male Lewis rats were used as recipients and Brown-Norway rats as donors, and the heterotopic transplants were done using ono＇s method with some modifications. The ascending aorta and pulmonary artery of donor were anastomosed end-to-side to the recipient abdominal aorta and inferior vena cava respectively. Results：48 heart transplantations were performed. We summarized surgical technique after sixteen rats of preliminary experiment. The successful rate was improved to 96.88% in 32 rats of formal transplantation.Time of cold ischemia,hot ischemia and rebeat were shorten significantly and operational time reduced to 60 min.Conclusion：To strengthen the protection of donor heart and perioperative treatment is the key point in the heart heterotopic transplantation in inbred strain rat.