Skp2、c-Myc和P27^(kip1)蛋白在大肠癌组织中的表达及相关性研究

Expression of Skp2,c-Myc and P27^(kip1) protein in colorectal cancer tissue and their correlation

目的:探讨Skp2、c-Myc和P27kip1蛋白在大肠癌组织中表达的意义以及相关性。方法:采用免疫组化SP法检测20例正常大肠粘膜、20例大肠腺瘤性息肉及60例大肠癌组织中Skp2、c-Myc和P27kip1蛋白表达情况,并分析与临床病理参数之间的关系,以及Skp2与c-Myc和P27kip1蛋白表达的相关性分析。结果:Skp2、c-Myc和P27kip1蛋白在大肠癌组织中阳性表达率分别为71.67%、66.67%和21.67%。随肿瘤细胞分化程度降低、浸润深度加深、病理分期延迟及伴有淋巴结的转移,P27kip1阳性表达率逐渐降低,Skp2阳性表达率逐渐升高。c-Myc过表达与浸润深度加深、病理分期延迟及伴有淋巴结的转移有关。三者的阳性表达率均与大肠癌患者的年龄和性别无关。大肠癌组织中Skp2与P27kip1蛋白表达呈负相关,Skp2与c-Myc蛋白表达呈正相关。结论:从正常大肠粘膜-大肠腺瘤性息肉-大肠癌过程中,Skp2、c-Myc和P27kip1蛋白表达发生相应变化,均参与大肠癌的发生和发展,其蛋白的检测可为判断大肠癌恶性进程和预后提供重要参考,同时也为大肠癌的靶向治疗提供理论依据。

Objective： To investigate the expression of Skp2,c-Myc and P27^kip1 protein, their association with clinicopathological features and the correlation between one and another. Method：Skp2,c-Myc and P27^kip1 protein were examined by using immunochistochemical staining method in 20 cases of normal coloreetal mucosa, 20 cases of colorectal adenomatous polyp and 60 cases of colorectal cancer,and analysed the relationship with clinicopathologic features and between Skp2 with c-Myc and P27^kip1 protein. Results：The positive rates of Skp2,c-Myc and P27^kip1 protein in colorectal cancer were 71.67% ,66.67% and 21.67% respectively.Moreover,the expression levels of Skp2 and P27^kip1 protein in colorectal cancer were significantly associated with the pathologic grade,invasion deep,clinical stage and lymph node metastasis,c-Myc associated with the invasion deep,clinical stage and lymph node metastasis,but were independent of age and sex. Besides,in colorectal cancer,the expression levels of Skp2 and P27^kip1 protein were inversely correlated, and the expression levels of Skp2 and c-Myc protein were positively correlated.Conclusions：The expression of Skp2,c-Myc and P27^kip1 protein are associated with occurrence and development in colorectal cancer through their alteration, which is very important for assessment of malignancy degree and prognosis in colorectal cancer, and detection cancer of their expression provides theoretical basis for targetting therapy of colorectal.