驯鹿狂蝇(Cephenemyia trompe)线粒体COI基因序列研究

Study of the mitochondrial Cytochrome oxidase I(COI)gene sequence of Cephenemyia trompe

利用分子生物学技术对驯鹿狂蝇蛆及其成蝇(Cephenemyia trompe)的线粒体COI基因种属特异性序列进行了研究。DNA核苷酸测序结果证实:中国内蒙古株驯鹿狂蝇蛆(C.trompeCIML)及其成蝇(C.trompe CIMA)线粒体COI种属特异性基因片段长度约为672bp～676bp。种系发生进化树分析显示:第三期幼虫与成蝇两者在核苷酸碱基序列上存在一定差异;它们与同种不同挪威株(C.trompe)同源性较为接近;与同属不同种(C.stimulator和C.ulrichii)同源性稍差;与不同属种的羊狂蝇(Oestrusovis)同源性较远。狂蝇科不同属间序列差异明显,差异性在18.6%～23%之间;鹿蝇属不同种间差异性在1.9%～9.9%之间;驯鹿狂蝇不同株间差异性为0.9%。说明生物线粒体COI基因核苷酸序列在一定程度上,可反映出种属及株间在进化上的差异性。

The mitochondrial cytochrome oxidase I （COI） gene sequence of larvae and adult of Cephenemyia trompe in reindeer for evolution by the molecular biology techniques. The results of DNA nucleotide sequence confirmed that the size of mitochondrial Cytochrome oxidase I gene （partial cds.） of larvae and adult of C. trompe from the reindeer in Inner Mongolia region of China was 672 bp～ 676 bp. The analysis of phylogenic evolution tree showed that there were a few differences for the nucleotide sequence between adults （C. trompe CIMA） and larvae 3 （C. trompe CIML） whose homology was near to C. trompe of Norway isolate （C. trompe L）and slightly far from C. stimulutor and C. ulrichii belongs to the same genus but different species. However, they were far from Oestrus ovis of being different genus on homology. There were obvious differences between different genus of Oestridae and variation within each genus ranged from 18.6 % to 23 %. Different species pairwise divergences of Cephenemyia ranged from 1.9 % to 9.9 % and intraspecific variation of C. trompe was 0.9 %. This study indicated that the nucleotide sequence of the mitochondrial Cytochrome oxidase I （COI） gene could reflect the phylogenetic relationships among genera, species and strains to some extent.