摘要
通过对南极冰藻L4(Chlamydomonnas L4)cDNA文库的筛选,克隆出一个具有5’和3’非编码区的40S核糖体蛋白S5(RPS5)全长基因序列。通过与GenBank中日本凋毛藻、拟南芥、果蝇、斑马鱼、非洲爪蟾、人等物种的同源基因或ESTs序列比对,得到了根据RPS5基因的序列相似性绘制的系统分类树,其符合传统的物种分类系统。证明了通过持家基因的一种——RPS5基因的序列相似性进行分子分类学研究具有可行性。
A full-length 40S ribosomal protein S5(RPS5) gene sequence was cloned from Antarctic ice microalgae Chlamydomonas L4. Having compared with the homologous gene or ESTs sequence of Griffithsia japonica, Arabidopsis thaliana , Drosophila melanogaster , Danio rerio , Xenopus laevis and Homo sapiens in the GenBank, the taxonomic system tree based on the comparability of RPS5 genes was protracted, and the system tree is consistent with the traditional species taxonomic system, which indicates that the molecular taxonomy study based on the sequence comparability of RPS5 genes is feasible.
出处
《海洋科学进展》
CAS
CSCD
北大核心
2005年第B12期51-54,共4页
Advances in Marine Science
基金
国家自然科学基金项目--南极冰藻抗冻蛋白的研究(40306027)
青岛市自然科学基金项目--极端环境下南极冰藻抗逆基因的研究(052-JC-56)
国家海洋局青年基金项目南极冰藻抗逆藻种的筛选和cDNA文库构建(2006103)