cDNA芯片检测耐药急淋患者细胞凋亡调控相关基因的差异表达

Detection of the differential expression of genes associated with apoptosis in the drug resistant ALL patiens with cDNA microarray

目的通过观察耐药/非耐药急性淋巴细胞性白血病(急淋)患者细胞凋亡调控相关基因的差异表达,探索多药耐药的分子病理机制。方法用TR Izo l抽提外周血标本总RNA,逆转录生成cDNA并标记后,与含有4 096个人类已知基因的cDNA表达谱芯片杂交,检测细胞凋亡调控相关基因在两者间的差异表达。结果耐药组与非耐药组有150个基因表达显著差异,包括耐药组83个基因低表达,67个基因高表达,其中凋亡调控相关基因有6个低表达,8个高表达。结论细胞凋亡调控相关基因表达异常可能是白血病多药耐药的分子病理机制之一。

[Objective] To explore the molecular pathological mechanism of multidrug resistance by detecting the differential expression of apoptosis associated genes between the multidrug resistant/non-resistant acute lymphocytic leukaemia （ALL） patiens. [Method] Omni-RNA was extracted with TRIzol from peripheral blood and reversely transcribed to cDNAs as hybridization probes which were hybridized with BioStar 4 096 cDNA microarray. Then the differentially expressed genes related to apoptosis between the two groups were analyzed. [Results] 150 genes expressed differently including 83 lower expression and 67 higher expression genes in multdrug resistant patiens group. Among the 150 genes,6 genes associated with apoptosis were up-regulated and 8 genes were down-regulated. [Conclusion] Genes associated with apoptosis were abnormally expressed may be one of molecular pathological mechanism of the multidrug resistant.