扶正消瘤颗粒抗卵巢癌作用的体外实验研究

In vitro Eexperimental Sstudy on Aanti-Oophoromamalignant tumor of ovary Eeffect of Fuzheng Xiaoliu Particles (Granula扶正消瘤颗粒)

目的:阐述扶正消瘤颗粒(CM)在体外的抗肿瘤作用。方法:制备含药血清,作用于体外培养的肿瘤细胞系SKOV-3,分为空白组(control)、CM组、顺铂组(DDP)和CM+DDP组。采用:MIT法检测其量效关系和时效关系,用TUNEL、透射电镜和流式细胞术(FCM)检测细胞凋亡的发生,用Westerblot检测PCNA、bcl-2和bax的表达。结果:MMT显示本方有一定量效关系和时效关系,浓度愈大,作用时间愈长,效果愈明显;TUNEL和透射电镜及FCM均提示了细胞凋亡的发生,而Westernblot的结果则显示了细胞凋亡的发生与bcl-2／bax比率的下调有关,使细胞增殖受到抑制。结论:扶正消瘤颗粒在体外有一定的抗肿瘤作用,并有增效之功。

Objective： To study anti-tumor effects of Fuzheng Xiaoliu ParticlesGranula （CM, 扶正消瘤颗粒 strengthen the body resisteance and resolve neoplasm） on tumor cells in vitro. Methods： Rats were divided into 4 groups randomly： control group, Fuzheng Xiaoliu Particles （扶正消瘤颗粒）（CM） group, cis-dichlorodiamine platinum （DDP） group and CMm＋DDP group. The Bblood serum including containing drugs were prepared were produced and was used applied to culture tumor cells SKOV-3 incubated in vitro. MTT method was used to detected Ddose-effect relationship and time-effect relationship was measured by MTT method. Apoptosis was observed by using TUNEL, transmission electron microscope and flow cytometry（FCM）. The expression of PCNA, bcl-2 and bax is were detected by Westerblot. Results： MMT results showed that this recipe possessed certain dose-effect relationship and time-effect relationship. With the higher the concentration increasing of CM and the longer the effect acting time applied lasting, the more pronounced effect wais shown obvious. TUNEL, transmission electron microscope and FCM results showed suggested the occurrence of apoptosis, which was related with the down-regulation. And the Westerblot results showed that apoptosis occurrence was related to decrease in the ratio of bcl-2 to bax as shown by Westerblot results, , which and the latter in- hibited cellproliferationcell proliferation. Conclusion： The Fuzheng Xiaoliu Particlesgranula （扶正消瘤颗粒） is showed certain anti-tumor effective to anti-tumor, as well as to enhance the effect.synergic action .