缺氧促进妊娠早期细胞滋养细胞凋亡机理研究

Altered Bcl-2 and Bax expression is associated with cultured first trimester human cytotrophoblasts apoptosis induced by hypoxia

目的探讨缺氧对妊娠早期细胞滋养细胞凋亡以及相关蛋白表达的影响。方法采用原代培养的妊娠早期细胞滋养细胞,用基因组DNA琼脂糖凝胶电泳和流式细胞术以及Western印迹等方法比较缺氧和正常氧体外培养后细胞凋亡以及凋亡相关蛋白Bcl-2和Bax表达的差异。结果妊娠早期的细胞滋养细胞缺氧培养24 h后电泳出现凋亡的典型特征DNA ladder,同时细胞凋亡率增加[(4.4±0.8)%和(17.4±3.0)%,P<0.05],并伴有Bcl-2蛋白表达减少和Bax蛋白表达增加(P<0.05)。结论缺氧促进妊娠早期细胞滋养细胞凋亡,伴有Bcl-2和Bax蛋白表达的改变。

Objective To investigate the effect of hypoxia on the apoptosis of cultured human cytotrophoblasts obtained at the first trimester and the expression of Bcl-2 and Bax, two apoptosis related proteins. Methods Human cytotrophoblasts of the first trimester were isolated and cultured under either normal or hypoxia conditions. Cellular apoptosis was monitored by agarose electrophoresis of genomic DNA and flow cytometry. The expression of Bcl-2 and Bax was determined by Western Blot. Results Cytotrophoblasts cultured for 24 h under hypoxia experienced significant apoptosis, as indicated by agarose electrophoresis of genomic DNA and flow cytometry. The apoptosis rate decreased significantly [（4.4±0.8）% vs （17.4±3.0）%, P〈0.05]. The expression of Bcl-2 reduced significantly, while the expression of Bax increased （P〈0.05）. Conclusions Hypoxia induces apoptosis in cultured first trimester cytotrophoblasts with altered Bcl-2 and Bax expression. Further study is needed to explore the role of cytotrophoblasts apoptosis in hypoxia-induced maternal and fetal diseases.