摘要
目的了解吉兰-巴雷综合征(GBS)相关空肠弯曲菌的WLAX基因序列特征,为进一步研究GBS相关空肠弯曲菌的分子致病机制打下基础。方法通过PCR方法对该基因进行扩增,将PCR产物克隆到质粒载体上,然后进行测序,将测序结果通过DNAstar软件进行比较和聚类分析。结果与非GBS相关空肠弯曲菌比较,GBS相关空肠弯曲菌的WLAX核苷酸序列发生变异的频率增大;菌株的核苷酸序列与全基因测序空肠弯曲菌NCTC11168比较存在差异;聚类关系反映了空肠弯曲菌具有一定的区域特征。结论GBS相关空肠弯曲菌中WLAX基因发生突变的概率明显增大,这些突变与GBS致病性的关系有待于进一步确定。
Objective To investigate the characteristic of sequences of WLAX gene in Campylobacter jejuni （ C. jejuni） strains. Methods WLAX gene and the neighbouring sequences were amplified by polymerase chain reaction （PCR）. The PCR products were cloned into the vectors of plasmid. The positive recombinants were sequenced and the results were processed by software DNAstar. Results The variation frequency of WLAX sequences in GBS-related C. jejuni was higher than that in non-GBS-related C. jejuni. The nucleotide sequences of WLAX gene in all the strains in the present study differed from that in genome sequencing strain NCTCll168. The phylogenic tree reflected the regional feature of C. jejuni. Conclusions The probability of sequence variation of WLAX in GBS-related C. jejuni is significantly higher than non-GBS-associated C. jejuni strains,the relation between the variation and GBSpathogenesis remains to be further confirmed.
出处
《基础医学与临床》
CSCD
北大核心
2006年第8期823-826,共4页
Basic and Clinical Medicine
基金
国家自然科学基金(30170052)
