转录因子FoxO1、FoxO3a在KKAy胰岛素抵抗糖尿病小鼠肌肉和肝组织中的表达

Transcription factors FoxO1 and FoxO3a expression on muscle and liver adipose tissue of KKAy diabetic mice

目的研究FoxO1和FoxO3 a在胰岛素抵抗糖尿病动物模型KKAy小鼠肌肉和肝中的表达,了解它们在胰岛素抵抗机制中所起的作用。方法对照组为16周龄C57BL小鼠7只,实验组为KKAy糖尿病小鼠7只;小鼠4周龄开始普通饲料喂养至12周龄,随后高脂饲料喂养4周,连续2次随机血糖≥16.7 mmol/L诊断为糖尿病。取股四头肌和肝加上组织裂解液B radford法测定蛋白浓度,W estern B lot方法检测肌肉和肝组织中FoxO1和FoxO3 a蛋白的表达。结果KKAy糖尿病小鼠FoxO1肝和肌肉中表达显著高于对照组小鼠;FoxO3 a肝表达对照组和糖尿病组之间没有差别,肌肉组织表达在糖尿病组显著高于对照组。结论FoxO1和FoxO3 a在胰岛素抵抗和糖尿病状态下表达显著增加,可能在胰岛素抵抗和糖尿病的发病机制中起重要作用。

Objective To detect the expression of FoxOl and FoxO3a in liver and muscle tissue from KKAy diabetic mice. Methods Study group consists of 7 KKAy diabetic mice until 12 weeks, thereafter fed high fat diet for 4 weeks. It was diagnosed as diabetes when blood glucose was more than 16. 7 mmol/L in two consequent weeks. Control group consists of 7 C57BL mice 16 week old. Dispatched the mice and took quadriceps of femoris muscle and liver tissue to detect the FoxOl and FoxO3a protein expression by Western blot with antibody. Results Liver and muscle FoxO1 expression was higher in KKAy diabetic mice than that in C57BL mice. FoxO3a expression was much higher in liver than in muscle in both groups. Muscle FoxO3a expression was higher in KKAy diabetic mice than that in control mice. Conclusion FoxOl and FoxO3a in liver and muscle expressed differently in diabetic mice and control mice. They may play a role in mechanisms of insulin resistance and type 2 diabetes mellitus.