摘要
应用抗脱氧雪腐镰刀菌烯醇单克隆抗体12D1建立了竞争间接ELISA方法,用于检测小麦、玉米中的脱氧雪腐镰刀菌烯醇(DON),最低检出限为20ng/mL,检测范围为20￣460ng/mL。用蒸馏水提取掺合DON(250￣4000ng/g)的小麦样品,回收率为82.1%￣96.6%,变异系数为0.6%￣7.1%。
A competitive indirect ELISA with anti-DON McAb for detecting deoxynivalenol in wheat and corn was developed. The detection limits was 20ng/mL and the linear range of the inhibition curve was 20-460ng/mL. In the detection of the samples of wheat and corn mixed with DON（250-4000ng/g）, extracted with water, the recovery rate of DON in the samples ranged from 82.1% to 96.6%, and the coefficients of variation was from 0.6% to 7.1%.
出处
《食品科技》
CAS
北大核心
2006年第8期222-224,共3页
Food Science and Technology
基金
"十五"国家重大科技专项"食品安全关键技术"课题(国科农社函[2002]130号)
