耐药性癫癎患者脑组织中热休克蛋白27相关蛋白1的表达

The expression of heat shock 27 000 associated protein 1 in the brains of drug-refractory epilepsy

目的研究热休克蛋白27相关蛋白1(heat shock 27 000 associated protein 1,HSPBAP1, GenBank:AK096705)在耐药性癫痫患者脑组织中的表达,探讨其在耐药性癫痫发病机制中的作用。方法从重庆医科大学附属第一医院神经内科建立的耐药性癫痫脑库中随机抽取36例耐药性癫痫患者术后脑组织,用荧光定量聚合酶链反应(FQ-PCR)和免疫组化法对HSPBAP1的表达进行检测,并与8例对照进行比较。结果耐药性癫痫患者脑组织中HSPBAP1 mRNA的相对表达量是对照组的34．11倍;HSPBAP1蛋白在耐药性癫痫患者颞叶(0．0507±0．0003,P<0．01)和海马(0．0488±0．0196,P<0．05)中的表达量,与对照组相同部位比较明显增加。结论耐药性癫痫患者脑组织中HSPBAP1表达增高,能抑制热休克蛋白27(HSP27)的神经元保护作用,可能是导致神经元凋亡及海马苔藓纤维发芽的一个重要原因。

Objective To study the expression of heat shock 27 000 associated protein 1 （ HSPBAP1, GenBank： AK096705 ） in the brain tissues of patients with drug-refractory epilepsy and discuss its function in the pathogenesis. Methods Fluorescent quantitative polymerase chain reaction （FQ-PCR） and immunohistochemistry were used to test the expression of HSPBAP1 in the surgically removed brain tissues of patients with drug-refractory epilepsy from the brain bank of our department （ n = 36 ） , and the results were compared with that of normal controls （ n = 8 ）. Results The relative expression of HSPBAP1 mRNA in the brains of patients with drug-refractory epilepsy was more than 34. 11 times that of controls, and HSPBAP1 protein expression was significantly increased in temporal lobe cortex （0. 0507 ±0. 0003, P 〈 0.01 ） and hippocampus （ 0. 0488 ±0. 0196, P 〈 0.05 ） of drug-refractory epilepsy as compared with controls in the same regions. Conclusion The results indicate that the overexpression of HSPBAP1 inhibits the neuroprotective effects of heat shock protein 27 （ HSP27 ） , resulting in neuron apoptosis and sprouting of hippocampal mossy fibers.