摘要
对利用微小毛霉摇瓶发酵合成凝乳酶的基本条件进行了初步研究，在质量分数为２％的麸皮水解液中，起始ｐＨ６．０，装液量３０ｍｌ／２５０ｍｌ三角瓶，３５℃，振荡速度１２０ｒ／ｍｉｎ条件下摇瓶发酵７２ｈ可达产酶高峰。可得凝乳酶５０００ｕ／ｇ麸皮。同时对微生物凝乳酶的工业生产可能性作了简要分析。
This paper was about preliminary lab research of the basic conditions on which rennase shaking flask fermentation by Mucor pusillus was studied. The maximun activity of the rennase was obtained with 72 h of incubution under conditions of 2% wheat bran (try wieght), initial pH6. 0,30ml of medium in 250ml of falsk, 35℃ and shaking speed of 120r/min. Yield of the rennase reached 5000u/g bran. Meanwhile, the production possibity of microbial rennase was analysed simply.
出处
《天津轻工业学院学报》
1996年第2期37-41,共5页
Journal of Tianjin University of Light Industry
