双色荧光原位杂交技术检测慢性淋巴细胞白血病IgH基因易位

Detection of IgH gene translocation in chronic lymphocytic leukemia by dual-color fluorescence in situ hybridization

目的了解慢性淋巴细胞白血病(CLL)中染色体14q32上IgH基因易位情况,探讨其与CLL疾病进展的关系。方法运用位于14q32上IgH基因两端的序列特异性DNA探针IGHC、IGHV和双色间期荧光原位杂交(FISH)技术对70例初发的B细胞CLL(B-CLL)患者的间期细胞进行IgH基因易位重排情况的检测。结果70例B-CLL中8例(11．4％)有IgH基因易位重排,其阳性细胞率为10．5％～53．0％之间,且IgH基因易位在不同性别、年龄和Binet分期中差异无统计学意义(P>0．05)。结论IgH基因异位在B-CLL中属易发事件,对B-CLL的发生和发展的作用有待进一步研究。FISH是一种在分析B-CLL中IgH基因易位异常方面较为快速、准确和敏感的方法。

Objective To investigate the translocation of IgH gene at chromosome 14q32 in chronic lymphocytic leukemia （CLL） and explore the relationship between the IgH gene translocation and progression of CLL Methods IGHC and IGHV DNA specific probes for IgH gene and interphase fluorescence in situ hybridization （FISH） were used for detecting the IgH gene translocation in 70 patients with newly diagnosed B cell CLL （B-CLL）. Results Out of the 70 patients, the IgH gene translocation was found in 8 patients （ 11.4% ） with the rate of IgH translocation cells ranging from 10. 5% to 53.0%. There was no significant difference in the IgH gene translocation either between two sexes or among ages or Binet stages （ P 〉 0. 05）. Conclusion The IgH translocation is a frequent event in B-CLL, and further investigation of the effect of the IgH translocation on the pathogenesis and progression of B-CLL is needed. FISH is a rapid, exact and sensitive technique in analysis of IgH gene translocation in CLL.