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一致-简并杂交寡核苷酸引物设计用于未知病原微生物检测的进展 被引量:2

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摘要 近年研究发现,暴发的“非典型性肺炎”,禽流感及四川发生的猪链球菌感染等要么是已发现的病原微生物家族中的新成员,要么是原来不感染人的动物病原体。因此,快速、及时、准确定性检测这些病原体,对防治疾病具有重要意义。
作者 谢珊 李金明
出处 《中华检验医学杂志》 CAS CSCD 北大核心 2006年第8期754-756,共3页 Chinese Journal of Laboratory Medicine
基金 国家自然科学基金资助项目(30371365)
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参考文献14

  • 1Sambrook J, Russell DW. Molecular Cloning. third edition,2001. http://www. MolecularCloning. com
  • 2Rose TM, Schultz ER, Henikoff JG. , et al. Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences. Nucleic Acids Res, 1998,26 : 1628-1635.
  • 3Rose TM, Henikoff JG. , Henikoff S. CODEHOP ( COnsensus- DEgenerate Hybrid Oligonucleotide Primer) PCR primer design. Nucleic Acids Res, 2003,31: 3763-37666.
  • 4Henikoff JG. , Henikoff S. Using substitution probabilities to improve position-specific scoring matrices. Comput. Appl. Biosci, 1996,12 : 135-143.
  • 5Rychlik W, Spencer WJ, Rhoads RE. Optimization of the annealing temperature for DNA amplification in vitro. Nucleic Acids Res, 1990,18:6409-6412.
  • 6Henikoff S, Henikoff JG. , Alford WJ, et al. Automated construction and graphical presentationofprotein blocks from unaligned sequences,Gene , 1995,163 : GC17-26.
  • 7Hermouet S, Souton CA, Rost TM, et al, Qualitative and quantitative analysis of human herpesviruses in chronic and acute B cell lymphocytic leukemia and in multiple myeloma. Leukemia, 2003,17 : 185-195.
  • 8Baines JE, McGovern RM, Persing D, et al . Consensus-degenerate hybrid oligonncleotide primers (CODEHOP) for the detection of novel papillomaviruses and their application to esophageal and tonsillar carcinomas. J Virol Methods, 2005 ,123:81-87.
  • 9Revankar SG, Fu J, Rinaldi MG, et al. Cloning and characterization of the lanosterol 14alpha-demethylase ( ERG11 ) gene in Cryptococcus neoformans. Biochem Biophys Res Commun, 2004 ,324:719-728.
  • 10Lourenco PM, Almeida T, Mendonca D, et al. Searching for nitrile hydratase using the Consensus-Degenerate Hybrid Oligonucleotide Primers strategy. J Basic Microbiol, 2004,44:203-214.

二级参考文献7

  • 1Rita RD, Timothy JS, David CB.Isolation and expression of homeobox genes from the embryonic chicken eye. Molecular Vision, 1997;3(10):7.
  • 2Timothy MR, Emili RS, Jorja GH, et al. Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequenes. Nucleci Acids Reseach, 1998;26(7):1628.
  • 3Boman HG. Peptide antibiotics and their role in innate immunity. Annu Rev Immunol,1995;13(1):61.
  • 4Stenger S, Hanson DA, Teitelbaum R, et al. An antimicrobial activity of cytolytic T cells mediated by granulysin. Science,1998;282(5386):121.
  • 5Timothy MR, Jorja GH, Stenven H. CODEHOP PCR primer design. Nucleic Acids Research, 2003;31(13):3763.
  • 6黄菁,乔传令,李瑄,邢建民.解毒酶基因的克隆及在大肠杆菌中的融合表达[J].Acta Genetica Sinica,2001,28(6):583-588. 被引量:6
  • 7杨华蓉,潘小玲,黄宁,吴琦,冯云,王伯瑶.人子宫颈粘液抗菌活性多肽的分离纯化[J].四川大学学报(医学版),2003,34(2):214-216. 被引量:5

共引文献25

同被引文献20

  • 1Lin Yuan-zhen Zhang Zhi-yi Lin Shan-zhi Zhang Qian Wang Xin.High Level Expression of Glucose-6-phosphate Dehydrogenase Gene PsG6PDH from Populus suaveolens in E. coli[J].Forestry Studies in China,2005,7(3):35-38. 被引量:5
  • 2夏瑞,陆旺金,李建国,杜娟.简并引物的程序化设计与荔枝HMGR基因片段的克隆[J].果树学报,2006,23(6):903-906. 被引量:16
  • 3侯林,姜丽娟,孙文静,张瑞锋,王家庆,赵欣涛,安家璐.中国卤虫actin基因的实时荧光定量PCR方法的建立和优化[J].辽宁师范大学学报(自然科学版),2006,29(4):466-469. 被引量:8
  • 4DEARDEN P,AKAM M.Developmental evolution:Axial patterning in insects Curr[J].Biol,1999,9(3):591-594.
  • 5LYNCHL Jeremy A,BRENTL Ava E,LEAF David S.Localized maternal Orthodenticle patterns anterior and posterior in the long germ wasp Nasonia[J].Nature,2006,439(9):728-732.
  • 6MIGUEL P,LOURENO L,ALMEIDA T.Searching for nitrile hydratase using the consensus-degenerate hybrid oligonucleotide primers strategy[J].Basic Microbiol,2004,44(3):203-214.
  • 7REGEARD C,MAILLARD J,HOLLIGER C.Development of degenerate and specific PCR primers for the detection and isolation of known and putative chloroethene reductive dehalogenase genes[J].Journal of Microbiological Methods,2004(56):107-118.
  • 8HENIKOFF S,HENIKOFF G J,ALFORD JW,et al.Automated construction and graphical presentation of protein blocks from unaligned sequences[J].Gene-combis Gene,1995,163(2):17-26.
  • 9HENIKOFF S,HENIKOFF G J,ALFORD JW,et al.Block+:A non-redundant database of protein alignment blocks derived from multiple compilations[J].Bioinformatics,1999,15(6):471-479.
  • 10ROSEM T,SCHULA R E,HENIKOFF G J.Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences[J].Nucleic Acids Research,1998,26(7):1628-1635.

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