HLAⅠ、Ⅱ类基因与肾综合征出血热重型与轻型的相关性研究

Study on relationship between HLAⅠ,Ⅱgenetic types and severity of hemorrhagic fever with renal syn-drome(HFRS)

目的探讨HLAⅠ、Ⅱ类基因多态性与肾综合征出血热(hemorrhagic fever with renal syn- drome,HFRS)患者临床特征的相关性。方法应用PCR-SSO(polymerase chain reaction using sequence- specific oligonucleotides)基因分型技术对中国北方地区56例汉族HFRS患者HLAⅠ、Ⅱ类基因频率分布进行了检测。结果HFRS重型组HLA-B35携带率为20％,HLA-B62及HLA-DR11携带率皆为15％,与轻型组比较差异皆具有统计学意义(P<0．05)。结论HLAⅠ类基因中HLA-B35、HLA-B62及HLAⅡ类基因中HLA-DR11与中国北方地区汉族人群HFRS患者病情严重性密切相关。

Objective To investigate the relationship between genetic polymorphisms of HLA Ⅰ、Ⅱ genes and clinical characters of patients with hemorrhagic fever with renal syndrome（ HFRS）. Methods HLA Ⅰ、Ⅱ genes were genotyped by PCR-SSO（ polymerase chain reaction using sequence-specific oligonucleotides） in the 56 patients with HFRS from the North China. The allele frequencies of HLAⅠ、Ⅱ genes were estimated by direct analysis. Results In the serious HFRS group, the allele frequencies of HLA-B35, HLA-B62 and HLA-DR11 were 20%, 15% and 15%, respectively. The three allele frequencies were significantly higher than those in the light group, and there was difference between the serious HFRS group and the light group（ P 〈 0.05 ）. Conduslon HLA-B35, HLA-B62 and HLA-DR11 might be related to the occurrence of serious HFRS in Noah China.