果胶酶产生菌ZH-g的原生质体形成与再生研究

Study on Protoplast Formation and Regeneration of Pectinase Producing Bacterial ZH-g

本文通过研究酶浓度、酶的作用时间、菌龄、菌体预处理等不同因素对原生质体形成与再生的影响,得出了果胶酶产生菌ZH-g原生质体制备和再生的最佳条件:加入0.6μg/ml的氨苄青霉素进行菌体预处理,选用对数生长期的菌株,以1.2mg/ml溶菌酶37℃保温处理2h,原生质体形成率为92%,再生率达50.7%。

The optimum conditions for preparation and regeneration of ZH-g protoplast were obtained after studying the effects of different factors on them. Under this circumstance-treating strains in log phase with concentration of 1.2mg/ml lysozyme for 2 hours, after pretreatment on the cells with 0.6μg/ml ampicillin, the protoplast formation is up to 92%, and regeneration reaches 50.7%.