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牛初乳中类胰岛素生长因子的提纯研究 被引量:3

Study on Purification of Bovine Colostrums Insulin-like Growth Factors
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摘要 本文研究了从牛初乳中用中空纤维超滤膜分离纯化牛初乳类胰岛素生长因子(IGF-I与IGF-Ⅱ),并利用放射免疫测定法(RIA)测定IGF-I与IGF-Ⅱ的浓度。结果表明:在碱性条件下超滤有利于提高类胰岛素生长因子的回收量。 The purification of bovine colostrums insulin-like growth factors with the dialysis and polyacrylonitrile hollow fiber ultra-filtration membrane was studied. The density of IGF-I and IGF-Ⅱ could be assayed through radio-immunoassay (RIA). The results showed that the recovery of insulin-like growth factors(IGFs) is optimal on alkaline diafiltration.
出处 《食品科学》 EI CAS CSCD 北大核心 2006年第8期144-148,共5页 Food Science
基金 江苏省教育厅自然科学基金专项项目(04KJD550027)
关键词 牛初乳 类胰岛素生长因子(IGFs) 超滤 提纯 bovine colostrums, insulin-like growth factors(IGFs) ultra-filtration purification
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  • 1魏辉俊,黄秉仁,蔡良琬.类胰岛素生长因子-Ⅰ的克隆、表达、分离纯化与活性检测[J].中国医学科学院学报,1997,19(5):342-345. 被引量:5
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  • 4Bang P,Eriksson U,Sara V,et al.Comparison of acid ethanol extraction and acid gel filtration prior to IGF-Ⅰ and radio-immunoassays:improvement of determination acid ethanol extractions by use of truncated IGF-Ⅰ as radio-ligand[J].Acta Endocrinology,1991,124:620-629.
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  • 6Kim L Hossner,Robert S Yemm.Improved recovery of insulin-like growth factors (IGFs) fiom bovine colostrums using alkaline diafiltration[J].Biotechnol Appl Biochem,2000,32:161-166.

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