摘要
本文采用吸光光度法,研究并优化了了铅与水溶性络合剂meso-四(4-磺酸苯基)卟啉(TPPS4)的显色条件,实验表明,在碱性条件(pH12)下,用邻二氮菲、硫氰酸铵、酒石酸钾钠作联合掩蔽剂,盐酸羟胺作辅助络合剂,铅与TPPS4需12min即可生成淡紫色络合物,该络合物的吸收峰为465nm,络合比为1:1。当铅含量在0～0.2μg/ml范围内时,萃取曲线符合比尔定律,用APDC/MIBK体系萃取样品中的铅离子,与TPPS4络合后比色测定几种食品中的铅,结果可行。
The color reaction of lead with meso-tetrakis （4-sulfophenyl） porphyrin （TPPS4） was studied in this paper by using spectrophotometric method. The results indicated that in the presence of hydroxylamine hydrochloride, with ortho-dinitrogenous ptenanthrene, NH4SCN and seignette saltat as masking agents, TPPS4 and lead is light purple in an alkaline solution of pH 12 at room temperature. A stable 1：1 （M：L） complex is formed. The Soret band of the complex is at 465nm, and Beer＇s law is obeyed in the range of 0-0.2μg of lead per ml. This method has been used to determine lead in some food with satisfactory results. Key words： lead; meso-tetrakis （4-sulfophenyl） porphyrin （TPPS4）; spectrophotometric method.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第8期217-220,共4页
Food Science
基金
河北省自然科学基金资助项目(203147)
关键词
铅
卟啉
分光光度法
lead
meso-tetrakis （4-sulfophenyl） porphyrin （TPPS4）
spectrophotometric method.
