固相微萃取技术及其在酒类风味成分分析上的应用

Application Review of Solid-phase Microextraction Technique for Aroma Analysis in Alcoholic Beverage

固相微萃取技术(SPME)是一种快速富集微量样品成分的预处理方法,可以和常用的分析检测仪器直接联用。由于具有快速、重现性好、无溶剂等优点,已广泛应用到许多领域内。本文论述了SPME的发展历程,重点介绍了固相微萃取的基本原理、影响萃取效果的因素及其在酒类风味成分分析中的应用;也分析比较了基于SPME的几种改进仪器(SPDE、SBSE、HSSE)的应用研究进展;同时也指出了该技术目前存在的一些问题和应用发展的前景。

Solid-phase microextraction （SPME） is a new sample preconcentration and enrichment technique for volatile compounds. The SPME technique can be used routinely in combination with gas chromatography （GC）, GC-mass spectrometry （GC-MS）, high-performance liquid chromatography （HPLC） or LC-MS. These methods using SPME techniques can save preparation time, solvent purchase and disposal costs, and improve the detection limits. This review summarizes the SPMEtechnique theories and influence factors for coupling with various analytical instruments and also the application for aroma analysis in alcoholic beverage, as well as for presenting the questions on SPME technique at present and its development foreground.