NQO1基因ns-cSNP与淮安汉族人群食管癌易感性的关系

Relationship between quinone oxidoreductasel gene ns-cSNP and genetic susceptibility of esophageal cancer

目的探讨依赖还原型辅酶Ⅰ/Ⅱ醌氧化还原酶1(quinone oxidoreductasel,NQO1)基因非同义编码 SNP(C609T、C465T、G406C)与淮安汉族人群食管癌易感性的关系。方法采用1:1配对的病例-对照研究,选择淮安地区原发性食管癌患者和对照者各106例。采用 PCR-RFLP 和 AS—PCR 技术检测研究对象的基因型,比较不同基因型与食管癌易感性的关系。结果在对照组与病例组中均未检测到 NQ01 406C/C 基因型;未发现 NQO1 CA65T 多态性与淮安汉族人群食管癌易感性的关系;携带 NQ01 609T 等位基因的个体与对照组相比,其患食管癌的易感性升高(OR=4.76,95%CI=1.064～3.397)。结论 NQ01 C609T 基因多态可能与食管癌的发生有关。

Objective To explore the relationship between quinone oxidoreductasel （NQO1） gene nonsynonymous cSNP and the genetic susceptibility of esophageal cancer. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and Allele-Specific PCR （AS-PCR） were employed to assess the polymorphism of NQO1 genes both in 106 patients with esophageal cancer and control subjects matched by age, gender and origin. Results It was shown that no C/C genotype was found at 406 of NQO1. The allelic frequency of NQO1 609T was significantly higher in patients with esophageal cancer than in the control subjects （P 〈 0. 005 ） and the individuals with 609T allelic genotype of NQO1 gene were at greater risk to develop esophageal cancer（ OR = 4. 76, 95% CI = 1. 064 - 3. 397 ）. But Individuals with mutant allele of NQO1 465 genotype did not show the rising risk of esophageal cancer. Conclusion The NQO1 C609T polymorphisms should likely be associated with the genetic susceptibility of esophageal cancer.