每日或间歇性大剂量补充硫酸亚铁对大鼠骨髓造血机能的影响

Effects of ferrous sulfate supplementation on bone marrow hemopoiesis in rats

目的比较不同方式大剂量补充硫酸亚铁对大鼠骨髓造血机能的影响。方法刚断乳的雌性 Wistar 大鼠,用铁缺乏饲料喂养至血红蛋白低于100g/L。按血红蛋白含量和体重分为铁缺乏对照组、每日小剂量补铁组、每周1次小剂量补铁组、每日大剂量补铁组和每周1次大剂量补铁组。补充期12周。实验结束时,取股骨骨髓,涂片染色,观察骨髓细胞形态学和骨髓可染色铁的状况。结果对照组大鼠骨髓细胞外可染色铁减少或缺失十分明显,细胞内铁主要以0型铁颗粒幼红细胞为主。各补充组大鼠骨髓中幼红细胞增生较明显,细胞外铁多数为3型以上,铁粒幼细胞的比例超过30%。从骨髓细胞增生分级看,所有大鼠骨髓细胞增生程度表现为增生活跃或增生明显活跃,属于正常骨髓的表现形式,没有其他诸如白血病等的表现。结论 4种补充方式大鼠的铁营养状况均得到显著改善;每日大剂量补充或每周1次大剂量补充3个月,并未发现有损骨髓的造血机能。

Objective To explore the influences of different dosage ferrous sulfate supplements on bone marrow hemopoiesis in rats. Methods Female weaning Wistar rats were fed with an iron deficient diet （ 〈 10 mg/kg diet） until the level of hemoglobin of rats was lower than 100 g/L. Rats （n = 50） were randomly divided into five groups according to the levels of hemoglobin and body weight, iron deficiency control （ ID）, daily low iron diet supplement （ LDs）, daily high iron diet supplement （ HDs）, weekly low iron supplement （ LWs）, and weekly high iron supplement （HWs）. Results After 12 weeks, bone marrow stainable iron was seldom in ID group, and ample in supplement groups. The proportions of iron staining of bone marrow smear in supplement groups were more than 30%. Bone marrow cells in all rats were hyperplastic or active hyperplastic. Conclusion Daily high iron supplement or once weekly high iron supplement were safe to bone marrow hemopoiesis in rats.