摘要
以特定方法筛得羟基脂肪酸聚酯（ＰＨＡＳ）产生株ＡＭＢ－００１，初步鉴定为产碱菌（Ａｌｃａｌｉｇｅｎｅｓ）属。经紫外线与亚硝基胍诱变，变异株ＡＵＮ－３９产聚羟丁酸（ＰＨＢ）和聚羟丁戊酸共聚物（ＰＨＢＶ）的产率分别为细胞干重的３５％和４７．５％．透射电镜观察表明ＡＵＮ－３９胞内积累的ＰＨＢ粒子可充满胞内空间。
Bacteria AMB-001 accumulating polyhyclroxyalkanoates (PHAs) was screened out through special procedure. and identified as the Genus Alcaligenes. The mutant AUN-39 was obtained after UV light and NTG mutagenesis, and found to synthesize PHB 35%and PHBV 47% of cell dry weight respectively. Transmittional Electronmicroscopy showed that PHB granules almost occupied the whole cellular plasmic space.
基金
国家自然科学基金
关键词
羟基脂肪酸聚酯
产生菌
选育
发酵微生物
Alcaligenes
Polyhydroxybutyrate (PHB)
Polyhydroxybutyrate-co-valerate (PHBV)
