聚合酶链反应对关节结核病诊断与鉴别诊断的临床价值

The clinical value of polymerose chain reaction assay in the diagnosis of joint tuberculosis and differentiation from other joint diseases

为探讨聚合酶链反应(PCR)对关节结核病诊断与鉴别诊断的临床价值,对30例关节结核和20例非关节结核标本分别采用PCR、抗酸染色镜检与分离培养法检测结核杆菌。结果30例关节结核标本中阳性检出率分别为:PCR法87%,镜检法0,培养法13%。经统计学处理,PCR与镜检及培养法对于结核杆菌的阳性检出率比较具有显著性差别(P<0.05)。20例非关节结核标本,镜检及培养法均阴性,PCR法阳性率10%。PCR法仅需要数小时,显著地缩短了检出时间。此表明PCR是一种敏感、特异、快速及简便的关节结核标本结核杆菌检测方法,对关节结核病的诊断与鉴别诊断具有极其重要的价值。

To evaluate the efficacy of PCR assay in the diagnosis of joint tuberculosis and differentiation from other joint diseases, the three methods of PCR protocol, standard light-microscope technique and standard culture technique were used to detect mycobacterium tuberculosis in samples obtained from 30 patients with joint tuberculosis and 20 patients without joint tuberculosis. In the group of 30 patients with joint tuberculosis, the positive rate was 87% in PCR protocol, 0 in standard light microscope technique and 13% in standard cultive technique. A marked difference was seen statistically (P<0.005). In the group of 20 patients without joint tuberculosis, 2 cases showed postitive in PCR protocol, none in the other methods. In conclusion, PCR protocol is a rapid、specific、sensitive and simple methold for detection of mycobacterium tuberculosis in samples of joint tuberculosis, and it is valuable in the diagnosis of joint tuber culosis and differentiating joint tuberculosis from other joint diseases.