亚硝基胍对泥鳅红细胞微核及核异常的诱发

Induction of micronuclei and nuclear anomalies in erythrocytes of loach by N-methyl-N'-nitro-N-nitrosoguanidine

研究了诱变剂对泥鳅红细胞微核和核异常的诱发作用，以寻求较为简便的检测水体中污染物对遗传物质的损害程度及毒理效应的方法。试验以亚硝基胍（ＭＮＮＧ）作为诱变剂，研究其不同浓度和染毒时间对泥鳅红细胞微核形成和核异常的影响。试验结果表明，在一定浓度范围内，微核细胞率与亚硝基胍浓度呈正相关；但当浓度过高时，微核细胞率反而降低。此外，研究还发现，随着亚硝基胍浓度的升高，微核细胞率出现高峰的时间也相应提前。从试验结果来看，微核测定法确是遗传毒理学试验中一个较为理想的监测手段。

Causing damages to the erythrocyte nucleus of loach Misgurnus anguillicaudatus by the chemical mutagen .N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) is reported in this paper. The test results indicate that MNNG could increase the frequency of micronuclei and nuclear anomalies in the erythrocytes. At certain range of MNNG concentration, the frequency of micronucleated erythrocytes shows a dosage-dependent elevation. However, it decreases with the concentration over a suitable range. In addition ,with the increase of the dosages,the high frequency of micronucleated erythrocytes appears also earlier.